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Related Experiment Videos

Poly(dA).poly(dT) forms very stable nucleosomes at higher temperatures

H L Puhl1, M J Behe

  • 1Department of Chemistry, Lehigh University, Bethlehem, PA 18015.

Journal of Molecular Biology
|February 3, 1995
PubMed
Summary

Poly(dA).poly(dT) can form nucleosomes, challenging previous beliefs. Its nucleosome formation ability increases with temperature, outcompeting other DNA sequences at higher heat.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Poly(dA).poly(dT), a synthetic DNA polymer, was historically considered resistant to nucleosome formation.
  • Previous research demonstrated nucleoprotein complex formation with authentic nucleosomes via low-salt exchange.
  • Competitive assays at 37°C indicated comparable reconstitution to heterogeneous DNA.

Purpose of the Study:

  • To investigate the temperature-dependent nucleosome formation of poly(dA).poly(dT).
  • To determine if poly(dA).poly(dT) can outcompete heterogeneous DNA for histone binding at varying temperatures.

Main Methods:

  • Utilizing competitive exchange assays at different temperatures.
  • Measuring the ability of poly(dA).poly(dT) to form nucleosomes as a function of temperature.

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  • Comparing histone binding affinity of poly(dA).poly(dT) against heterogeneous-sequence DNA.

    • Main Results:

    • Poly(dA).poly(dT) exhibits enhanced nucleosome formation with increasing temperature.
    • The homopurine.homopyrimidine polymer's ability to form nucleosomes is temperature-dependent.
    • At elevated temperatures, poly(dA).poly(dT) successfully outcompetes heterogeneous DNA for histone binding.

    Conclusions:

    • The conformation of poly(dA).poly(dT) influences its nucleosome-forming capacity.
    • Temperature is a critical factor in the nucleosome assembly of synthetic DNA polymers.
    • Poly(dA).poly(dT) can be effectively incorporated into nucleosomes under specific thermal conditions.