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Purification of lysozyme from human neutrophils, and development of an ELISA for quantification in cells and plasma
K Lollike1, L Kjeldsen, H Sengeløv
1Department of Hematology, National University Hospital, Rigshopitalet, Copenhagen, Denmark.
Leukemia
|January 1, 1995
Abstract:
Lysozyme was purified from exocytosed granule material from PMA-stimulated human neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and plasma.
