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A modified cell block technique for fine needle aspiration cytology
F A Zito1, C D Gadaleta, C Salvatore
1Division of Anatomical Pathology and Cytology, National Cancer Research Institute of Bari, Italy.
Acta Cytologica
|January 1, 1995
Summary
This study presents a modified cell block technique for fine needle aspiration (FNA) cytology, achieving high diagnostic accuracy. While valuable for immunohistochemistry, the technique
Area of Science:
- Cytopathology
- Histopathology
- Oncology
Background:
- Fine needle aspiration (FNA) is a crucial diagnostic tool.
- The cell block technique enhances FNA sample processing.
- Optimizing FNA processing is vital for accurate diagnoses.
Purpose of the Study:
- To describe a modified cell block technique for FNA specimens.
- To evaluate the diagnostic accuracy of the modified cell block technique.
- To assess the utility of the technique for immunohistochemical staining.
Main Methods:
- A modified cell block technique was applied to 406 FNA samples from 333 patients.
- Specimens were fixed in 4% buffered paraformaldehyde.
- Conventional histochemical and immunohistochemical stains were utilized.
Main Results:
- Histologic verification was achieved in 67 cases.
- The overall diagnostic accuracy was 97% (95% sensitivity, 100% specificity).
- The technique is particularly useful for immunohistochemical analysis.
Conclusions:
- The modified cell block technique offers high accuracy for FNA cytology.
- Its primary limitation is the increased processing time compared to smears.
- The method is recommended when immunohistochemical staining is required for definitive diagnosis.