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Neutrophil and monocyte beta 2-integrin expression in maternal and fetal blood
B Thilaganathan1, G Makrydimas, N Plachouras
1Harris Birthright Research Centre for Fetal Medicine, King's College Hospital School of Medicine, London, United Kingdom.
Objective:
The aim of this study was to investigate fetal and maternal leukocyte beta 2-integrin expression throughout pregnancy.
Study Design:
In a cross-sectional study of 173 fetuses at 18 to 40 weeks' gestation, 114 women with normal singleton pregnancies at 6 to 40 weeks, and 25 adult volunteers, flow cytometry was used to determine neutrophil and monocyte expression of beta 2-integrins.
Results:
The surface density of beta 2-integrins on fetal neutrophils and monocytes increased significantly with gestation, except for the expression of fetal monocyte CD11b, which did not change. There were no significant changes in maternal beta 2-integrin expression with gestation. During the third trimester fetal expression of CD18, CD11b, and CD11c on neutrophils and CD11b on monocytes was lower, and maternal expression of CD11a on neutrophils and CD18, CD11a and CD11c on monocytes was higher than were the control adult values.
Conclusions:
These data demonstrate that pregnancy is associated with changes in the expression of maternal beta 2-integrins and illustrate the pattern of immunophenotypic maturation of fetal beta 2-integrins on neutrophils and monocytes.