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Related Experiment Videos

Direct effects of thrombin on myocyte contractile function

R B Hird1, F A Crawford, R Mukherjee

  • 1Division of Cardiothoracic Surgery, Medical University of South Carolina, Charleston 29425.

The Annals of Thoracic Surgery
|February 1, 1995
PubMed
Summary

Thrombin directly impairs heart muscle cell function and response to beta-adrenergic stimulation. This suggests thrombin may contribute to heart dysfunction after cardiopulmonary bypass surgery.

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Area of Science:

  • Cardiovascular Physiology
  • Biochemistry

Background:

  • Cardiopulmonary bypass (CPB) elevates thrombin levels.
  • Thrombin influences various cell types, suggesting potential cardiac effects.

Purpose of the Study:

  • To investigate the direct impact of thrombin on left ventricular myocyte contractile function.
  • To assess thrombin's effect on myocyte beta-adrenergic responsiveness.

Main Methods:

  • Isolated adult dog left ventricular myocytes were studied using videomicroscopy.
  • Myocyte function was measured at baseline and with increasing thrombin concentrations (1-10 U/mL).
  • The effect of thrombin inhibition (hirudin) and beta-adrenergic stimulation (isoproterenol) was evaluated.

Main Results:

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  • Thrombin reduced myocyte shortening in a dose-dependent manner (e.g., 43% reduction at 2 U/mL).
  • Hirudin completely blocked thrombin's negative effects on myocyte function.
  • Thrombin significantly blunted beta-adrenergic responsiveness, reducing shortening velocity by 25%.

    • Conclusions:

    • Thrombin directly impairs ventricular myocyte contractility and beta-adrenergic responsiveness.
    • Elevated thrombin levels post-CPB may contribute to transient left ventricular dysfunction.