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Tissue-specific expression of Ran isoforms in the mouse

E E Coutavas1, C M Hsieh, M Ren

  • 1Department of Biochemistry, New York University Medical Center, New York 10016.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|October 1, 1994
PubMed
Summary

This study investigates Ran gene expression in mice, revealing tissue-specific isoforms, particularly in the testis. These findings help explain the diverse roles of Ras-related nuclear proteins in cellular functions.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Ran genes encode small nuclear GTPases crucial for cell cycle and nucleocytoplasmic transport.
  • Previous research on Ran proteins used cell-free systems, yeast, and cultured mammalian cells.
  • This study focuses on characterizing Ran gene expression patterns in vivo within the mouse model.

Purpose of the Study:

  • To investigate the patterns of Ran gene expression in various mouse tissues.
  • To identify and characterize different Ran gene transcripts and their tissue-specific distribution.
  • To explore the potential functional implications of identified Ran isoforms.

Main Methods:

  • Serum starvation and refeeding experiments in mouse 3T3 cells to observe transcriptional regulation.

Related Experiment Videos

  • Northern blotting and reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze mRNA expression.
  • Analysis of testis-derived cDNA clones to identify specific Ran transcripts.

    • Main Results:

    • Serum starvation suppressed Ran gene transcription in 3T3 cells; mRNA reappeared upon refeeding.
    • A single Ran mRNA species was found at low levels in most adult mouse somatic tissues.
    • The testis exhibited abundant Ran mRNA, including larger transcripts, and two distinct cDNA-derived isoforms: one identical to human Ran/TC4 and a variant 94% identical.
    • The Ran/TC4-identical isoform was expressed in both somatic tissues and testis, while the variant isoform was testis-specific.

    Conclusions:

    • Ran gene expression is regulated by cellular conditions like serum availability.
    • The mouse testis expresses unique, tissue-specific Ran isoforms.
    • The existence of these Ran isoforms may explain the varied functions attributed to Ras-related nuclear proteins.