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Immunization of mice with proteins reacted with malonic dialdehyde (MDA): comparison between autologous and
O Martinon1, J P Poulet, Y Chancerelle
1Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Abstract:
In previous experiments, rabbits were injected with heterologous proteins reacted with MDA, and produced antibodies cross-reacting with other MDA-modified proteins (MPr), but not with the corresponding native ones (Pr). It was concluded that these antibodies (AbAIP) recognized epitopes including 1-amino-3-imino-propene (AIP) bridges resulting from reactions of MDA with primary amino groups of proteins. In the present work, mice were injected with autologous MDA-modified albumin (MAI) or with heterologous MPr. Mice immunized with MAI developed an immune response leading to an increased production of AbAIP, which clearly indicates that such a response may occur even with an autologous MPr.
Insights
Mice produced antibodies against modified proteins, even when the proteins were their own. This suggests the immune system can recognize self-proteins altered by malondialdehyde (MDA).
Area of Science:
- Immunology
- Biochemistry
- Protein Modification
Background:
- Previous studies showed rabbits produced antibodies against heterologous malondialdehyde-modified proteins (MPr). These antibodies cross-reacted with other MPr but not native proteins (Pr).
- Antibodies were concluded to recognize 1-amino-3-imino-propene (AIP) bridges formed by malondialdehyde (MDA) reacting with protein amino groups.
Purpose of the Study:
- To investigate if an immune response can be generated against autologous MDA-modified proteins.
- To determine if mice immunized with autologous MDA-modified albumin (MAI) produce antibodies recognizing MDA-modified proteins (MPr).
Main Methods:
- Mice were immunized with either autologous MDA-modified albumin (MAI) or heterologous MPr.
- The resulting immune response, specifically the production of antibodies against 1-amino-3-imino-propene (AbAIP), was analyzed.
Main Results:
- Mice immunized with autologous MAI demonstrated an increased production of AbAIP.
- This indicates that the immune system can mount a response against self-proteins modified by MDA.
Conclusions:
- An immune response, including AbAIP production, can occur even when using autologous MPr for immunization.
- This finding has implications for understanding immune reactions to modified self-proteins.