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Perfluorodecalin emulsion tested for biocompatibility in macrophages by means of a magnetometric method
M Koester1, J Lutz, A J Augustin
1Dept. of Physiology, University of Wuerzburg, Germany.
Abstract:
The effect of a stable perfluorodecalin (FDC) emulsion, based on addition of 0.2-0.5% (W/V) of perfluoroperhydrophenanthrene and 4% egg yolk lecithin was tested for its influence on Kupffer cells in vivo by means of a magnetometric procedure in rats. It was also compared with the effect of a perfluoro-chemical emulsion (PFC) of the first generation (FluosolR-DA, FDA). While doses of 0.1 g/kg body weight of both PFCs caused a small increase of activity in these cells (as measured by an increase in a ratio factor r after magnetization), doses of 1 g/kg b.wt. led to a significant retardation of intracellular movements after FDC and FDA for two and four days, respectively. A dose of 3 g/kg b.wt. of FDC effected a significant depression for 8 days, whereas after the same dose of Fluosol-DA the intracellular motility remained depressed for as long as 32 days.
Insights
A novel perfluorodecalin (FDC) emulsion and first-generation perfluorochemical (PFC) emulsion were tested for their effects on rat Kupffer cells. Higher doses of both emulsions temporarily reduced Kupffer cell activity, with FDC showing a shorter duration of effect.
Area of Science:
- Biomedical Engineering
- Immunology
- Toxicology
Background:
- Perfluorochemical (PFC) emulsions are used in biomedical applications, including as blood substitutes.
- Kupffer cells are resident macrophages in the liver, playing a crucial role in immune responses.
- Understanding the in vivo effects of novel PFC emulsions on immune cells is vital for assessing their safety and efficacy.
Purpose of the Study:
- To evaluate the influence of a stable perfluorodecalin (FDC) emulsion on rat Kupffer cells in vivo.
- To compare the effects of FDC emulsion with a first-generation PFC emulsion (Fluosol-DA, FDA).
- To assess the dose-dependent and time-dependent effects of these emulsions on Kupffer cell intracellular motility.
Main Methods:
- A magnetometric procedure was employed to assess Kupffer cell activity in rats.
- Rats were administered varying doses (0.1 g/kg, 1 g/kg, 3 g/kg body weight) of FDC and FDA emulsions.
- Intracellular movements of Kupffer cells were measured at different time points post-administration.
Main Results:
- Low doses (0.1 g/kg) of both FDC and FDA caused a minor increase in Kupffer cell activity.
- Higher doses (1 g/kg) led to a significant retardation of intracellular movements for 2 days (FDC) and 4 days (FDA).
- A high dose (3 g/kg) of FDC significantly depressed Kupffer cell activity for 8 days, while FDA caused depression for 32 days.
Conclusions:
- Both FDC and FDA emulsions can modulate Kupffer cell activity in a dose-dependent manner.
- The FDC emulsion demonstrated a less prolonged effect on Kupffer cell intracellular motility compared to FDA at higher doses.
- These findings suggest differential immunomodulatory effects of PFC emulsions, impacting their potential clinical applications.
