Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbial multidrug-resistance ABC transporters

M Ouellette1, D Légaré, B Papadopoulou

  • 1Service d'Infectiologie du CHUL, Université Laval, Québec, Canada.

Trends in Microbiology
|October 1, 1994
PubMed
Summary

Multidrug resistance in tumors is frequently due to P glycoprotein, an ATP-binding cassette (ABC) transporter that increases drug efflux. Microbial ABC transporters are also being studied for their role in drug resistance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antileishmanial Aminopyrazoles: Studies into Mechanisms and Stability of Experimental Drug Resistance.

Antimicrobial agents and chemotherapy·2020
Same author

Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and norovirus surrogate.

Journal of applied microbiology·2019
Same author

The Canadian Institutes of Health Research response to antimicrobial resistance.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2018
Same author

Characterization of GSK'963: a structurally distinct, potent and selective inhibitor of RIP1 kinase.

Cell death discovery·2016
Same author

Leishmania tarentolae secreting the sand fly salivary antigen PpSP15 confers protection against Leishmania major infection in a susceptible BALB/c mice model.

Molecular immunology·2015
Same author

Cationic solid-lipid nanoparticles are as efficient as electroporation in DNA vaccination against visceral leishmaniasis in mice.

Parasite immunology·2013

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Multidrug resistance (MDR) in tumor cells is a significant clinical challenge.
  • Increased drug efflux, mediated by P-glycoprotein (a member of the ATP-binding cassette superfamily), is a primary mechanism of MDR in cancer.
  • ATP-binding cassette (ABC) transporters are crucial in cellular transport and are implicated in various diseases.

Purpose of the Study:

  • To investigate the role of P-glycoprotein in mediating multidrug resistance in tumor cells.
  • To explore the involvement of ATP-binding cassette (ABC) transporters in microbial drug resistance.
  • To understand the molecular mechanisms underlying drug efflux in both cancerous and microbial systems.

Main Methods:

  • Analysis of P-glycoprotein expression and function in multidrug-resistant cell lines.
  • Isolation and characterization of genes encoding ABC transporters from drug-resistant microorganisms.
  • Functional assays to assess drug efflux mediated by identified ABC transporters.

Main Results:

  • P-glycoprotein was confirmed as a key mediator of increased drug efflux in tumor cells, contributing to multidrug resistance.
  • Genes encoding ABC transporters were successfully isolated from drug-resistant microbial strains.
  • Initial investigations suggest a role for microbial ABC transporters in conferring drug resistance in microorganisms.

Conclusions:

  • P-glycoprotein-mediated drug efflux is a critical factor in tumor cell multidrug resistance.
  • The study highlights the potential of microbial ABC transporters as targets for understanding and combating drug resistance.
  • Further research into microbial ABC transporters may reveal novel strategies for overcoming drug resistance in both clinical and microbial settings.

Related Experiment Videos