Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replication initiation. A new controller in Escherichia coli

T A Baker1

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

Current Biology : CB
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteasomes in lungs from organ donors and patients with end-stage pulmonary diseases.

Physiological research·2014
Same author

Effect of ragi and LXXX-lactate-producing cultures on enteric pathogens in a rice-based weaning food.

World journal of microbiology & biotechnology·2014
Same author

Carbapenem-resistant Acinetobacter baumannii infections after organ transplantation.

Transplant infectious disease : an official journal of the Transplantation Society·2009
Same author

Transient hydroxyl formation from water on oxygen-covered Au(111).

The Journal of chemical physics·2008
Same author

Performance requirements of physically strenuous occupations: validating minimum standards for muscular strength and endurance.

Ergonomics·2004
Same author

Comparative architecture of transposase and integrase complexes.

Nature structural biology·2002
Same journal

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same journal

Sub-second fluctuations between top-down and bottom-up modes distinguish diverse human brain states.

Current biology : CB·2026
Same journal

Queen bees offload pesticide burden to eggs when social buffering is overwhelmed.

Current biology : CB·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
See all related articles

A newly discovered protein, SeqA, regulates DNA replication in Escherichia coli by binding to active replication origins. SeqA acts as a multifaceted negative regulator, controlling replication initiation.

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • DNA replication is a fundamental process in all living organisms.
  • Replication origins are specific DNA sites where replication begins.
  • Regulation of DNA replication is crucial for maintaining genomic stability.

Purpose of the Study:

  • To identify and characterize novel factors involved in the regulation of DNA replication initiation in Escherichia coli.
  • To elucidate the function of the newly discovered SeqA protein in controlling replication origin activity.

Main Methods:

  • Genetic analysis of Escherichia coli mutants.
  • Biochemical assays to study protein-DNA interactions.
  • Molecular cloning and characterization of the SeqA gene.

Related Experiment Videos

Main Results:

  • A novel protein, designated SeqA, was identified as a key factor in sequestering recently active replication origins.
  • SeqA was shown to be a multifaceted negative regulator of replication initiation.
  • The study provides evidence for SeqA's role in preventing over-initiation of DNA replication.

Conclusions:

  • SeqA plays a critical role in the regulation of DNA replication initiation in Escherichia coli.
  • The discovery of SeqA provides new insights into the complex mechanisms governing DNA replication.
  • SeqA represents a potential target for understanding and manipulating bacterial growth.