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Related Experiment Videos

Hyperammonaemia in relation to high-intensity exercise duration in man

D A Sewell1, M Gleeson, A K Blannin

  • 1Department of Physical Education, University of Warwick, Coventry, UK.

European Journal of Applied Physiology and Occupational Physiology
|January 1, 1994
PubMed
Summary

Intense exercise triggers adenine nucleotide (AN) degradation, indicated by increased plasma ammonia. This degradation correlates with high plasma lactate levels, suggesting a critical pH threshold during fatigue.

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Area of Science:

  • Exercise Physiology
  • Biochemistry

Background:

  • Adenine nucleotide (AN) degradation occurs during intense exercise, nearing fatigue.
  • Plasma ammonia concentration ([NH3]) is an indicator of AN degradation.

Purpose of the Study:

  • To compare plasma ammonia concentration ([NH3]) with plasma lactate concentration ([lactate]) during intense exercise.
  • To measure plasma glutamine concentration ([Gln]) pre- and post-exercise.

Main Methods:

  • Nine healthy subjects performed intense treadmill exercise (30-210s at 5.6 m.s-1) with varying inclines.
  • Blood samples were analyzed for plasma [lactate], [NH3], and [Gln].

Main Results:

  • Plasma [NH3] indicated varying degrees of AN degradation.

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  • A significant increase in AN degradation was observed when plasma [lactate] reached approximately 14 mmol.l-1.
  • Data support a critical intramuscular pH threshold stimulating AN degradation.

    • Conclusions:

    • Intense exercise leads to AN degradation, linked to increased plasma ammonia and lactate.
    • A critical intramuscular pH may stimulate AN degradation during exercise-induced fatigue.
    • Reduced phosphocreatine rephosphorylation kinetics and increased ADP may contribute to AN degradation.