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A simple approach to intracytoplasmic sperm injection
S H Atiee1, T B Pool, J E Martin
1Fertility Center of San Antonio, Texas.
Objective:
To describe a simple injection apparatus and method for performing intracytoplasmic sperm injection in a clinical IVF program.
Design:
A prospective clinical trial of intracytoplasmic sperm injection.
Setting:
A private office-based fertility program.
Patients:
Five couples undergoing IVF-ET with intracytoplasmic sperm injection as a treatment for male factor infertility.
Interventions:
Intracytoplasmic sperm injection was performed at room temperature (23.5 to 24.5 degrees C) in a simple zwitterion-buffered medium.
Main Outcome Measures:
Fertilization rates, cleavage rates, clinical pregnancy rates, implantation rates.
Results:
Intracytoplasmic sperm injection was performed on 44 fresh oocytes from five patients. Twenty-three oocytes fertilized (52.3%) and 22 zygotes cleaved (95.7%). Three of five patients became pregnant (60%), resulting in the live birth of one normal male infant, one continuing singleton pregnancy, and one continuing twin gestation (46XX, 46XY). The implantation rate was 23.5%.
Conclusion:
Intracytoplasmic sperm injection can be performed successfully in a simple medium at room temperature using commercially available microtools.