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Cryptic plasmids from Lactobacillus helveticus and their evolutionary relationship

D Pridmore1, T Stefanova, B Mollet

  • 1Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.

FEMS Microbiology Letters
|December 15, 1994
PubMed
Summary

Researchers identified three cryptic plasmids in Lactobacillus helveticus, revealing conserved non-coding DNA sequences. These plasmids utilize distinct replication genes, suggesting evolutionary acquisition from Gram-positive bacteria.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Lactobacillus helveticus is a key probiotic bacterium.
  • Cryptic plasmids are small, extrachromosomal DNA elements found in bacteria.
  • Understanding plasmid biology is crucial for bacterial genetics and biotechnology.

Purpose of the Study:

  • To identify and sequence cryptic plasmids from Lactobacillus helveticus.
  • To analyze the structural homology and genetic elements of these plasmids.
  • To investigate the evolutionary origins of plasmid replication genes.

Main Methods:

  • Plasmid DNA isolation and sequencing.
  • Bioinformatic analysis of DNA sequences.
  • Comparative genomics to identify homologous regions and replication genes.

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Main Results:

  • Three distinct cryptic plasmids were identified and sequenced.
  • High sequence identity (80-90%) was observed in large portions of non-coding plasmid regions.
  • Different plasmids utilized distinct replication genes, indicative of horizontal gene transfer.

Conclusions:

  • Lactobacillus helveticus cryptic plasmids share conserved non-coding sequences.
  • Replication genes appear to have been acquired independently from various Gram-positive bacterial sources.
  • Evidence suggests a remnant of foreign replication gene integration into a plasmid.