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Related Experiment Videos

Insertion sequence IS2 near the gene for prophage lambda excision

E Mosharrafa, W Pilacinski, J Zissler

    Molecular & General Genetics : MGG
    |August 10, 1976
    PubMed
    Summary
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    Expression plasmid with a very tight two-step control: Int/att-mediated gene inversion with respect to the stationary promoter.

    Gene·2001

    Researchers characterized a new lambda phage variant, lambdabi2cI857S7, with an IS2 DNA insertion. This mutant aids studies on IS2 recombination and its impact on lambda phage integration and excision.

    Area of Science:

    • Molecular Biology
    • Microbial Genetics
    • Bacteriophage Research

    Background:

    • Bacteriophage lambda is a well-studied model organism for genetic research.
    • Prophage integration and excision are critical steps in the lambda life cycle.
    • IS elements are mobile genetic elements with potential roles in genome evolution.

    Purpose of the Study:

    • To characterize a novel lambda phage variant, lambdabi2cI857S7, containing an IS2 insertion.
    • To investigate the location and orientation of the IS2 insertion within the lambda genome.
    • To assess the potential utility of this mutant for studying IS2 functions and their effects on lambda integration/excision.

    Main Methods:

    • Genetic characterization of the lambdabi2cI857S7 prophage variant.

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  • DNA sequencing to identify the insertion element and its precise location.
  • Analysis of the insertion's orientation relative to lambda genes.

    • Main Results:

    • A specific lambda prophage variant, lambdabi2cI857S7, was identified and characterized.
    • The insertion was confirmed to be the IS2 element.
    • IS2 was found in antipolar orientation II, upstream of thexis gene at 61.6%lambda.

    Conclusions:

    • The lambdabi2cI857S7 mutant provides a new tool for investigating IS2 DNA.
    • This variant can be used to study the influence of IS2 on lambda phage integration and excision processes.
    • Further research using this mutant may elucidate recombination functions associated with IS2.