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Related Experiment Videos

N-terminal sequence of phage lambda repressor

K Beyreuther, B Gronenborn

    Molecular & General Genetics : MGG
    |August 10, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Researchers purified lambda repressor from E. coli, determining the N-terminal sequence. This sequence contains key residues crucial for the repressor

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Lambda repressor controls the lysogenic cycle in bacteriophage lambda.
    • Understanding repressor-operator interactions is key to phage biology.

    Purpose of the Study:

    • To determine the N-terminal amino acid sequence of the lambda repressor.
    • To investigate the role of the N-terminal region in DNA binding.

    Main Methods:

    • Purification of lambda repressor from a high-expression E. coli strain.
    • Automated Edman degradation for N-terminal sequencing.

    Main Results:

    • The N-terminal sequence of fifty residues was determined.
    • This region comprises 43% of the repressor's arginine and lysine residues.

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  • Genetic data suggests this N-terminal region is vital for operator-DNA binding.

    • Conclusions:

    • The N-terminal region of lambda repressor is rich in basic residues.
    • This region likely plays a significant role in the repressor's interaction with operator DNA.