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DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect
C U Kirchgessner1, C K Patil, J W Evans
1Department of Radiation Oncology, Stanford University School of Medicine, CA 94305.
Abstract:
Severe combined immunodeficient (SCID) mice are deficient in a recombination process utilized in both DNA double-strand break repair and in V(D)J recombination. The phenotype of these mice involves both cellular hypersensitivity to ionizing radiation and a lack of B and T cell immunity. The catalytic subunit of DNA-dependent protein kinase, p350, was identified as a strong candidate for the murine gene SCID. Both p350 and a gene complementing the SCID defect colocalize to human chromosome 8q11. Chromosomal fragments expressing p350 complement the SCID phenotype, and p350 protein levels are greatly reduced in cells derived from SCID mice compared to cells from wild-type mice.
Insights
Severe combined immunodeficient (SCID) mice lack B and T cell immunity due to a DNA repair defect. The catalytic subunit of DNA-dependent protein kinase, p350, is identified as the defective gene in SCID mice.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Severe combined immunodeficient (SCID) mice exhibit cellular hypersensitivity to ionizing radiation and lack B and T cell immunity.
- SCID is characterized by a deficiency in a recombination process essential for DNA double-strand break repair and V(D)J recombination.
Purpose of the Study:
- To identify the genetic defect responsible for the SCID phenotype in mice.
- To investigate the role of the DNA-dependent protein kinase catalytic subunit (p350) in SCID.
Main Methods:
- Genetic analysis and complementation studies were performed.
- Chromosomal mapping was used to localize the SCID gene.
- Protein expression levels were compared between SCID and wild-type mice.
Main Results:
- The catalytic subunit of DNA-dependent protein kinase, p350, was identified as a strong candidate for the SCID gene.
- The p350 gene and a gene complementing the SCID defect colocalize to human chromosome 8q11.
- Chromosomal fragments expressing p350 complemented the SCID phenotype, and p350 protein levels were significantly reduced in SCID mouse cells.
Conclusions:
- The p350 gene defect is responsible for the SCID phenotype in mice.
- This finding provides a molecular basis for understanding SCID and potential therapeutic strategies.