Related Experiment Videos
Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line
S P Lees-Miller1, R Godbout, D W Chan
1Department of Biological Science, University of Calgary, Alberta, Canada.
Science (New York, N.Y.)
|February 24, 1995
Abstract:
The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.