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Phenotypic analysis of yellow fever virus derived from complementary DNA
R S Marchevsky1, J Mariano, V S Ferreira
1Fundacao Oswaldo Cruz, Instituto de Tecnologia em Imunobiologicos, Departamento de Bioquimica e Biologia Molecular, Rio de Janeiro, Brazil.
The American Journal of Tropical Medicine and Hygiene
|January 1, 1995
Summary
This study characterized yellow fever (YF) virus derived from cDNA. The experimental YF viruses showed favorable safety profiles, comparable to existing vaccines, supporting further research into YF virus virulence.
Area of Science:
- Virology
- Vaccinology
- Molecular Biology
Background:
- Phenotypic characterization of yellow fever (YF) virus is crucial for understanding virulence and developing new vaccines.
- The YF 17D vaccine strain holds potential as a carrier for heterologous protective epitopes.
Purpose of the Study:
- To produce and phenotypically characterize YF virus generated from cDNA clones.
- To evaluate the neurovirulence and safety of experimental YF viruses in comparison to a control vaccine strain.
Main Methods:
- Yellow fever virus was generated from 17D cDNA clones via RNA transfection of chicken embryo fibroblasts (YFiv5.2/SL).
- Experimental viruses were passaged in embryonated eggs (YFiv5.2/VL) following vaccine manufacturing methods.
- Neurovirulence safety tests in monkeys were conducted, yielding quantitative clinical and histologic scores.
Main Results:
- Experimental YF viruses (YFiv5.2/SL and VL) demonstrated favorable histologic scores compared to the 17DD control.
- Slightly increased clinical neurovirulence was noted for YFiv5.2 relative to the 17DD virus.
- The observed neurovirulence did not preclude the use of YFiv5.2 for mapping YF virus virulence determinants.
Conclusions:
- The characterized YF viruses derived from cDNA exhibit a safety profile suitable for further investigation.
- These findings support the utility of YFiv5.2 for mapping YF virus virulence and attenuation factors.
- The study validates the methodology for generating and characterizing YF virus from cDNA for research and vaccine development.