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Molecular typing of Actinomyces pyogenes isolates
V Guérin-Faublée1, D Decoret, A Kodjo
1Laboratoire de Microbiologie et Immunologie, Ecole Nationale Vétérinaire de Lyon, Marcy l'étoile, France.
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|August 1, 1994
Summary
Molecular typing of Actinomyces pyogenes strains using ribotyping proved effective for epidemiological studies. This method offers high discriminatory power, unlike SDS-PAGE or endonuclease analysis, and revealed no link between strain types and clinical origins.
Area of Science:
- Microbiology
- Molecular Biology
- Epidemiology
Background:
- Actinomyces pyogenes is a significant pathogen requiring accurate strain differentiation for epidemiological tracking.
- Previous molecular characterization methods for A. pyogenes have limitations in discriminatory power or interpretability.
Purpose of the Study:
- To evaluate different molecular techniques for characterizing clinical isolates of Actinomyces pyogenes.
- To assess the utility of these methods as epidemiological markers.
Main Methods:
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein profiling.
- Restriction endonuclease analysis of total DNA.
- Ribotyping following DNA digestion with Bst EII or Sma I.
Main Results:
- SDS-PAGE protein profiles were insufficient for distinguishing between A. pyogenes isolates.
- Restriction endonuclease analysis provided differentiation but yielded complex, difficult-to-interpret profiles.
- Ribotyping demonstrated high discriminatory power and proved to be a valuable epidemiological marker.
Conclusions:
- Ribotyping is a superior method for molecular typing of Actinomyces pyogenes clinical isolates compared to SDS-PAGE and restriction endonuclease analysis.
- No correlation was found between the identified molecular types and the clinical sources of the isolates, suggesting broad applicability or common transmission routes.