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Simultaneous determination of vitamins D3, E and K1 and retinyl palmitate in cattle plasma by liquid chromatography
D B Gomis1, V J Escotet Arias, L E Fidalgo Alvarez
1Departamento de Química Física y Analítica, Universidad de Oviedo, Spain.
Abstract:
A reversed-phase high performance liquid chromatographic method is described for the simultaneous determination of vitamins D3, E and K1 and retinyl palmitate in plasma. Narrow-bore columns are recommended because this alternative provides a good separation efficiency, plus greater economy and sensitivity. Detection limits for individual vitamins range from 0.42 to 2.8 ng. All vitamins were separated in less than 9 min. Recovery studies showed good results for all solutes (88.8-100.3%) and the intra-day coefficients of variations ranged from 1.0 to 4.5%. This method permits the simple determination of fat-soluble vitamins using 1 ml of cattle heparinized plasma.