[ABO genotyping of fingerprints by the PCR-RFLP method]

DNA typing of the ABO blood groups from single fingerprints was examined. Genomic DNAs were extracted from fingerprints on slide glasses by a simple method with NaI and N-lauroyl-sarcocinate, and then subjected to the PCR (Polymerase chain reaction). Two PCR fragments spanning positions 258 and 700 of the cDNA sequence of the ABO locus were amplified. Then, the PCR products were digested with a restriction enzyme, Kpn-1 or Msp-1, for each site. The digested PCR products were analyzed by electrophoresis on polyacrylamide gels for RFLP (Restriction-fragment length polymorphism). Bands on the gel were visualized by silver staining. Genotyping from single fingerprints after treatment with aluminium powder was also possible by these techniques.