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Rat liver chromatin phospholipids

E Albi1, M Mersel, C Leray

  • 1Istituto di Patologia Generale, Università di Perugia, Italy.

Lipids
|October 1, 1994
PubMed
Summary
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Phospholipids in chromatin are native, not from nuclear membrane contamination. Analysis of phospholipid (PL) composition in isolated chromatin revealed distinct profiles, suggesting endogenous origin.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Phospholipids (PL) are crucial components of cellular membranes.
  • Their presence in isolated chromatin raises questions about native incorporation versus nuclear membrane contamination.

Purpose of the Study:

  • To investigate whether phospholipids found in isolated chromatin originate from the nuclear membrane or are native components.
  • To determine the phospholipid and fatty acid composition of chromatin-associated phospholipids.

Main Methods:

  • Isolation of hepatocyte nuclei.
  • Radioiodination of phospholipids in isolated nuclei using the lactoperoxidase method.
  • Quantification of radioactivity in nuclear membranes versus extracted chromatin.
  • Analysis of phospholipid and fatty acid composition in chromatin.

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Main Results:

  • Only a small fraction (0.08-0.6%) of radioactivity was found in chromatin, while 97% remained in nuclear membranes.
  • Chromatin-associated phospholipids included phosphatidylethanolamine (10%), phosphatidylserine (22%), phosphatidylinositol (19%), phosphatidylcholine (14%), and sphingomyelin (35%).
  • An enrichment of polyunsaturated fatty acids was observed in chromatin-associated sphingomyelin.

Conclusions:

  • The phospholipid composition of isolated chromatin is unlikely to be due to contamination from the nuclear membrane.
  • Data suggests that phospholipids associated with chromatin are likely native components.