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Molecular cloning of Chlamydia trachomatis 26K protein expressed in Escherichia coli
M Del Pezzo1, M Rosiello, M Miloso
1Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina, Università degli Studi Federico II, Napoli, Italy.
The New Microbiologica
|October 1, 1994
Abstract:
Molecular genetics appears to be the most promising approach to understanding the biology and pathology of Chlamydia. This report focuses on the cloning and the protein expression of a DNA fragment from Chlamydia trachomatis DK20 chromosome. Results of hybridization experiments suggest that this sequence is specifically present within chlamydial DNA. The coding capacity of this DNA fragment is supported by the expression of a 26,000 m.w. peptide, in an Escherichia coli maxicell system.