Puncture perfusion of small tissue pieces for scanning electron microscopy

Small tissue pieces for scanning electron microscopy were punctured, before fixation, with a needle and perfused through the needle with Ringer solution. This puncture perfusion sufficiently eliminated blood and tissue fluid from the tissue and was of excellent value in cleaning the small samples which were hardly cannulated for vascular irrigation. Resin injection by this needle puncture method reproduced not the tissue spaces but, almost exclusively, the blood vessels.