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Related Experiment Videos

A human protein selected for interference with Ras function interacts directly with Ras and competes with Raf1

L Han1, J Colicelli

  • 1Department of Biological Chemistry, UCLA School of Medicine 90024.

Molecular and Cellular Biology
|March 1, 1995
PubMed
Summary

Researchers identified Rin1, a human protein that directly interacts with Ras proteins, particularly when Ras is bound to GTP. This interaction suggests Rin1 may function as a Ras effector or regulator in mammalian cells.

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Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Yeast Genetics

Background:

  • Human protein overexpression can disrupt the Ras signal transduction pathway in yeast.
  • Functional interference occurs at the effector level, downstream of activating mutations.

Purpose of the Study:

  • To identify and characterize human proteins that interact with Ras.
  • To elucidate the mechanism of Ras-protein interactions in yeast and mammalian systems.

Main Methods:

  • In vivo and in vitro experiments using yeast Saccharomyces cerevisiae and human H-Ras.
  • Analysis of protein-protein interactions using Ras mutants (effector and dominant negative).
  • Competition assays with known Ras effectors like Raf1.

Main Results:

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  • A human cDNA clone (99) encodes a protein, named Rin1, that directly binds yeast Ras2p and human H-Ras.
  • Rin1-Ras interaction is enhanced by GTP-bound Ras and is specific to functional Ras proteins.
  • Rin1 shares an interaction profile with known Ras effectors (Raf1, adenylyl cyclase) and competes with Raf1 for H-Ras binding.
  • Rin1 localizes to the plasma membrane, similar to H-Ras.

Conclusions:

  • Rin1 is a novel Ras-interacting protein identified through functional studies in yeast.
  • Rin1 exhibits characteristics of a Ras effector or regulator in mammalian cells.
  • Rin1's plasma membrane localization suggests a role in Ras-mediated signaling at the cell surface.