Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Habenula lesions decrease the responsiveness of dorsal raphe serotonin neurons to cocaine

J M Paris1, K A Cunningham

  • 1Department of Pharmacology and Toxicology, University Texas Medical Branch, Galveston 77555-1031.

Pharmacology, Biochemistry, and Behavior
|November 1, 1994
PubMed
Summary

Cocaine’s depressant effects on serotonin (5-HT) neurons in the dorsal raphe nucleus (DR) depend on an intact habenula. Short-term habenula lesions attenuated cocaine’s inhibitory response, suggesting habenula involvement in this circuit.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Covid-19 interface with drug misuse and substance use disorders.

Neuropharmacology·2021
Same author

Ear type in sheep is associated with the MSRB3 locus.

Animal genetics·2020
Same author

A de novo germline mutation of KIT in a white-spotted Brown Swiss cow.

Animal genetics·2020
Same author

Identification of two TYRP1 loss-of-function alleles in Valais Red sheep.

Animal genetics·2019
Same author

Incubation of cocaine cue reactivity associates with neuroadaptations in the cortical serotonin (5-HT) 5-HT2C receptor (5-HT2CR) system.

Neuroscience·2016
Same author

Variation within the serotonin (5-HT) 5-HT₂C receptor system aligns with vulnerability to cocaine cue reactivity.

Translational psychiatry·2014

Area of Science:

  • Neuroscience
  • Pharmacology
  • Serotonergic Systems

Background:

  • The median and dorsal raphe nuclei (MR and DR) are primary sources of forebrain serotonin (5-HT) innervation.
  • The medial and lateral habenula provide significant input to the midbrain, potentially forming a negative feedback loop.
  • The role of the habenula in mediating cocaine's effects on 5-HT neurons remains unclear.

Purpose of the Study:

  • To investigate whether intact habenula nuclei are necessary for cocaine's depressant effects on dorsal raphe (DR) 5-HT neuronal activity.
  • To determine the time course of habenula involvement in cocaine's neurochemical actions.

Main Methods:

  • Extracellular recordings of single 5-HT neurons in the DR of male rats.
  • Bilateral radiofrequency lesions of the habenula complex were performed 1-4 hours (short-term) or 7 days (long-term) prior to recordings.

Related Experiment Videos

  • Cocaine dose-response curves were generated to assess inhibitory effects (ID50).

    • Main Results:

    • Short-term habenula lesions significantly attenuated cocaine's inhibitory effect on DR 5-HT neurons, increasing the ID50 from 0.68 mg/kg to 2.5 mg/kg.
    • Short-term lesions reduced the number of 5-HT neurons in the DR but did not affect their firing rates.
    • Long-term habenula lesions showed no significant differences in cocaine's effects or 5-HT neuron characteristics compared to controls.

    Conclusions:

    • The inhibitory effects of cocaine on DR 5-HT neuronal activity are partially dependent on habenula function.
    • The habenula appears to play a transient role in modulating cocaine's impact on the serotonergic system.
    • These findings highlight the habenula's involvement in the neural circuitry underlying cocaine's neuropharmacological actions.