Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

alpha 2-8 Sialic acid polymers: size, structure, and compositional analysis

G Ashwell1, W K Berlin, O Gabriel

  • 1National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Analytical Biochemistry
|November 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nonequilibrium rovibrational energy distributions of hydrogen isotopologues in an expanding plasma jet.

The Journal of chemical physics·2010
Same author

Foam formation on an Austrian-Hungarian lowland river: reasons, methods and solutions.

Water science and technology : a journal of the International Association on Water Pollution Research·2009
Same author

Sink or source? - The effect of hydrology on phosphorus release in the cultivated riverine wetland Spreewald (Germany).

Water science and technology : a journal of the International Association on Water Pollution Research·2008
Same author

Formation and relaxation of rovibrationally excited H2 molecules due to plasma-surface interaction.

Physical review. E, Statistical, nonlinear, and soft matter physics·2008
Same author

Discussion of an environment quality standard based assessment procedure for permitting discharge.

Water science and technology : a journal of the International Association on Water Pollution Research·2007
Same author

Nitrogen fluxes on catchment scale: the influence of hydrological aspects.

Water science and technology : a journal of the International Association on Water Pollution Research·2006
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

New assays determine alpha 2-8 linked polysialic acid chain length, composition, and structure on cell surface glycoproteins. These methods rapidly analyze critical cell surface structures for glycoprotein research.

Area of Science:

  • Biochemistry
  • Glycobiology
  • Analytical Chemistry

Background:

  • Cell surface glycoproteins play critical roles in cellular processes.
  • Alpha 2-8 linked polysialic acid (PSA) is a key post-translational modification on many critical glycoproteins.
  • Understanding PSA structure and composition is vital for elucidating its biological functions.

Purpose of the Study:

  • To develop a series of variable assay procedures for analyzing alpha 2-8 linked polysialic acid chains.
  • To provide overlapping information on the size, structure, and composition of PSA on cell surface glycoproteins.
  • To enable rapid and facile determination of PSA characteristics.

Main Methods:

  • Utilized sequential periodate and borohydride modification of terminal sialic acid residues.

Related Experiment Videos

  • Developed three variable assay procedures for comprehensive PSA analysis.
  • Leveraged advances in instrumentation for enhanced analytical capabilities.

    • Main Results:

    • Established methods for determining polysialic acid chain length.
    • Enabled simultaneous identification of N-acetylneuraminic acid, N-glycolyneuraminic acid, and KDN.
    • Provided qualitative distinction between reducing and nonreducing polymers.
    • Allowed determination of the number of PSA chains bound to glycoproteins.

    Conclusions:

    • The described assay procedures offer a rapid and facile method for analyzing alpha 2-8 linked polysialic acid.
    • These methods provide comprehensive information on PSA size, structure, and composition.
    • The assays are applicable to a wide variety of cell surface glycoproteins, aiding in their functional studies.