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3-Aminobenzamide protects cells from UV-B-induced apoptosis by acting on cytoskeleton and substrate adhesion
W Malorni1, R Rivabene, E Straface
1Dipartimento di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.
Abstract:
3-aminobenzamide (3-ABA) is an inhibitor of poly-(ADP-ribose)-polymerase, an enzyme involved in numerous subcellular processes, including cell death. Recently, a target effect of the drug on some cytoskeletal elements has also been described (Malorni et al., Biochem. Biophys. Res. Commun. 202: 915-922, 1994). In this study we evaluated the ability of 3-ABA to interfere with UV-B ray-induced apoptosis in cells selected for their cytoskeletal features and their different capability to adhere to the substrate. Human melanoma (M14) and epithelial (A431) cell lines and murine primary fibroblastic cultures (MFC) were studied. Our results indicate that cytoskeleton is indeed an important cellular target of 3-ABA, which can prevent apoptotic cell death by UV-B through a specific effect on the adhesion properties of the cells. Indeed, an inverse correlation was observed between sensitivity to UV-B-induced apoptosis (M14 > A431 > MFC) and substrate adhesion (MFC > A431 > M14). The potential relevance of these observations to understand the possible relationships among apoptosis, cytoskeletal functions and substrate adhesion is discussed.
Insights
3-aminobenzamide (3-ABA) prevents UV-B induced cell death by affecting cell adhesion properties. This poly-(ADP-ribose)-polymerase inhibitor targets the cytoskeleton, influencing apoptosis sensitivity.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- 3-aminobenzamide (3-ABA) inhibits poly-(ADP-ribose)-polymerase (PARP), an enzyme crucial for cellular processes like cell death.
- Previous research suggests 3-ABA affects cytoskeletal elements.
Purpose of the Study:
- To investigate 3-ABA's ability to interfere with UV-B induced apoptosis.
- To examine the role of cytoskeletal features and substrate adhesion in UV-B sensitivity.
Main Methods:
- Utilized human melanoma (M14) and epithelial (A431) cell lines, along with murine primary fibroblastic cultures (MFC).
- Assessed the impact of 3-ABA on UV-B induced apoptosis in relation to cellular cytoskeletal features and substrate adhesion capabilities.
Main Results:
- Cytoskeleton is identified as a key cellular target of 3-ABA.
- 3-ABA prevents UV-B induced apoptosis by modulating cell adhesion properties.
- An inverse correlation exists between UV-B induced apoptosis sensitivity and substrate adhesion (M14 > A431 > MFC for sensitivity; MFC > A431 > M14 for adhesion).
Conclusions:
- 3-ABA's mechanism in preventing UV-B induced apoptosis involves targeting the cytoskeleton and altering cell adhesion.
- Findings highlight the interplay between apoptosis, cytoskeletal function, and substrate adhesion.