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Stable expression of the cDNA encoding the feline CD8 alpha gene
M R Pecoraro1, Y Kawaguchi, M Okita
1Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
The Journal of Veterinary Medical Science
|October 1, 1994
Abstract:
The stable expression of the alpha chain of the feline cytotoxic T cell differentiation antigen (fCD8 alpha) on Crandell feline kidney cells (CRFK) was carried out by using an expression vector which contains the Rous sarcoma virus long terminal repeat and a neo resistant gene. After three rounds of cloning under G418 selection for over two months, the expression of the feline polypeptide was detected by human monoclonal antibody OKT8.