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Updated: Aug 14, 2026
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
Published on: April 22, 2015
Immunocharacterization of H-2Kb-tsA58 transgenic mouse hippocampal neuroepithelial cells
T R Kershaw1, F Rashid-Doubell, J D Sinden
1Neurodegenerative Diseases Research Group, Institute of Psychiatry, De Crespigny Park, London, UK.
Abstract:
From dissected fragments of embryonic H-2Kb-tsA58 transgenic mouse hippocampal neuroepithelium, we have derived a population of rapidly proliferating, nestin-positive conditionally immortal hippocampal neuroepithelial cells. Treatment with dibutyryl cAMP in non-permissive culture conditions resulted in cessation of cell division and differentiation of the precursor cells into neuronal or glial phenotypes.
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