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Updated: Aug 14, 2026
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PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early
C Breton1, A Chaboud, E Matthys-Rochon
1E.N.S. Lyon, Reconnaissance Cellulaire et Amélioration des Plantes-CNRS-INRA, France.
Abstract:
One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. Calmodulin expression was confirmed throughout maize embryogenesis at the mRNA, amplified cDNA and protein levels. Sequence analysis suggests a maize origin for both clones and negligible nucleotide changes linked to PCR. This library is the first described for early plant embryos and represents a breakthrough to isolate genes involved in embryo differentiation.
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