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Updated: Aug 30, 2026

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
08:07

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Published on: February 17, 2016

Down-regulation of c-myc oncogene during NGF-induced differentiation of neuroblastoma cell lines

J Chen1, T Liu, A H Ross

  • 1Department of Pathology, Peking Union Medical College Hospital, CAMS & PUMC, Beijing.

Chinese Medical Sciences Journal = Chung-Kuo I Hsueh K'O Hsueh Tsa Chih
|September 1, 1994
PubMed

Insights

The study found that down-regulating the c-myc oncogene is crucial for nerve growth factor (NGF)-induced differentiation in neuroblastoma cells. Overexpression of c-myc may contribute to neuroblastoma development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Developmental Biology

Background:

  • Neuroblastoma is a pediatric cancer with a potential link to the myc oncogene family.
  • Nerve growth factor (NGF) has been shown to induce differentiation in some neuroblastoma cell lines.

Purpose of the Study:

  • To investigate the role of N-myc and c-myc oncogenes during NGF-induced differentiation of neuroblastoma cells.
  • To understand the gene regulation mechanisms underlying neuroblastoma differentiation.

Main Methods:

  • Hybridization of N-myc and c-myc cDNA probes with RNA from neuroblastoma cell lines before and after NGF treatment.
  • In situ hybridization to assess oncogene expression in differentiated cells.

Main Results:

  • Neuroblastoma cell lines exhibited inverse expression of N-myc and c-myc, with some exceptions.
  • NGF treatment led to the down-regulation of the c-myc oncogene in differentiated neuroblastoma cells.
  • Reduced c-myc expression correlated with morphological differentiation and was confirmed by in situ hybridization.

Conclusions:

  • Down-regulation of the c-myc oncogene appears to be a critical event in NGF-induced neuroblastoma differentiation.
  • Overexpression of the c-myc oncogene may play a role in the initiation and progression of neuroblastoma.

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