Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new rDNA repeat unit in human Giardia

J A Upcroft1, A Healey, P Upcroft

  • 1Queensland Institute of Medical Research, Brisbane, Australia.

The Journal of Eukaryotic Microbiology
|November 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving antenatal detection of small-for-gestational-age fetus: economic evaluation of Growth Assessment Protocol.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2022
Same author

Lifestyle information and commercial weight management groups to support maternal postnatal weight management and positive lifestyle behaviour: the SWAN feasibility randomised controlled trial.

BJOG : an international journal of obstetrics and gynaecology·2019
Same author

POPPIE: protocol for a randomised controlled pilot trial of continuity of midwifery care for women at increased risk of preterm birth.

Trials·2019
Same author

Potential Disadvantages of Overcentralization of Organ Recovery Centers: Response to Marsolais et al.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
Same author

Factors influencing attendance at structured education for Type 1 diabetes in south London.

Diabetic medicine : a journal of the British Diabetic Association·2017
Same author

Spontaneous spinal epidural haematoma mimicking meningitis in a 2-year-old child--a case report and literature review.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2013

Genetic analysis of a new human Giardia duodenalis strain reveals significant differences in its ribosomal DNA (rDNA) repeat unit compared to previously reported strains, impacting gene sequences and spacer length.

Area of Science:

  • Molecular biology
  • Parasitology
  • Genetics

Background:

  • Giardia duodenalis is a significant human intestinal parasite.
  • Ribosomal DNA (rDNA) repeat units are crucial for understanding genetic diversity within species.
  • Previous studies have characterized G. duodenalis rDNA, but variations exist.

Purpose of the Study:

  • To characterize the rDNA repeat unit of a newly identified human Giardia duodenalis strain.
  • To compare this new rDNA unit with previously reported G. duodenalis rDNA sequences.
  • To identify genetic variations, including changes in rRNA genes and spacer regions.

Main Methods:

  • DNA sequencing of the rDNA repeat unit.
  • Analysis of small subunit ribosomal RNA (SSrRNA) and large subunit ribosomal RNA (LSrRNA) genes.

Related Experiment Videos

  • Restriction enzyme digestion to identify new sites.
  • Comparative sequence analysis of gene and spacer regions.

    • Main Results:

    • The new G. duodenalis rDNA repeat unit exhibits significant differences from previously reported strains.
    • Twelve base-pair changes were identified within the SSrRNA gene (490 bp analyzed).
    • New restriction enzyme sites were found in the LSrRNA gene.
    • The rRNA genes maintained the same overall length, but the spacer region was 76 bp longer.
    • A boundary within the spacer region showed varying homology (50% near LSrRNA, 80% toward SSrRNA) and contained two copies of a 78 bp repeat.

    Conclusions:

    • The genetic variability in rDNA repeat units highlights the diversity within Giardia duodenalis populations.
    • These findings contribute to a better understanding of the genetic makeup of human-infecting G. duodenalis strains.
    • Further research is needed to determine the functional or epidemiological implications of these rDNA variations.