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Related Experiment Videos

Update on bladder smooth-muscle physiology

R M Levin1, A J Wein, R Buttyan

  • 1Division of Urology, Hospital of the University of Pennsylvania, Philadelphia.

World Journal of Urology
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

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Bladder distension causes significant growth and altered function, involving DNA synthesis and gene expression changes. Obstruction-induced bladder hypertrophy reverses upon removal, indicating opposing processes regulated by gene activity.

Area of Science:

  • Urology
  • Cell Biology
  • Physiology

Background:

  • The urinary bladder exhibits significant mass increases and functional alterations in response to various stresses like distension and outlet obstruction.
  • These changes are associated with increased DNA synthesis and alterations in gene expression.

Purpose of the Study:

  • To investigate the cellular and molecular mechanisms underlying bladder hypertrophy and regression following outlet obstruction.
  • To elucidate the role of gene expression in regulating bladder mass and function.

Main Methods:

  • Induction of bladder distension and outlet obstruction in experimental models.
  • Measurement of bladder mass, DNA synthesis ([3H]-thymidine uptake), and gene expression (heat-shock protein-70, growth factors, oncogenes).
  • Autoradiography to localize DNA synthesis within bladder tissues.

Related Experiment Videos

  • Induction of apoptosis and functional assessment after obstruction removal.

    • Main Results:

    • Bladder distension and outlet obstruction led to increased bladder mass, DNA synthesis (initially urothelium, then connective tissue), and altered contractile responses.
    • Acute obstruction increased expression of heat-shock protein-70, basic fibroblast growth factor, N-ras, and c-myc, while decreasing transforming growth factor-beta.
    • Obstruction removal induced apoptosis, increased transforming growth factor-beta, decreased basic fibroblast growth factor, and reversed bladder dysfunction.

    Conclusions:

    • Bladder hypertrophy following outlet obstruction involves complex changes in DNA synthesis and gene expression.
    • The regression of bladder mass after obstruction removal represents an opposing process, also governed by specific gene expression patterns.
    • These findings highlight the dynamic nature of bladder tissue remodeling in response to mechanical stress and its regulation by molecular pathways.