Related Experiment Videos
Quantitative analysis of DNA aberrations amplified by competitive polymerase chain reaction using capillary
A W Kuypers1, J P Meijerink, T F Smetsers
1Department of Internal Medicine, University Hospital Nijmegen, Netherlands.
Abstract:
We compared the use of capillary electrophoresis (CE) in a polymer network with the use of slab gel electrophoresis for the quantitative analysis of polymerase chain reaction (PCR)-amplified DNA samples. We quantified residual lymphoma cells carrying a translocation between chromosomes 14 and 18, in consecutive patient peripheral blood samples that were amplified by competitive PCR. For CE analysis we used a 4% linear polyacrylamide network. Results show that the calculated number of translocations in patient samples using both analyses were comparable. We conclude that CE is a sensitive, non-radioactive, fast and accurate method for quantitation of competitive PCR products.