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Related Experiment Videos

Bacterial structure and functional relation to abscess formation

A O Tzianabos1, A B Onderdonk, D L Kasper

  • 1Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115.

Infectious Agents and Disease
|October 1, 1994
PubMed
Summary
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The Bacteroides fragilis capsular polysaccharide complex (CPC) has dual roles in sepsis. Its charged groups promote abscess formation but also protect against infection, mediated by T cells.

Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • The capsular polysaccharide complex (CPC) from Bacteroides fragilis displays unique biological activities.
  • These activities include both promoting and preventing abscess formation in experimental models.

Purpose of the Study:

  • To investigate the structural basis for the dual biologic properties of the B. fragilis CPC.
  • To elucidate the T cell-dependent immune mechanisms underlying these properties.

Main Methods:

  • Analysis of the chemical structure of CPC polysaccharides (PS A and PS B).
  • Chemical modification of charged groups within the polysaccharides.
  • Assessment of abscess promotion and prevention in a rat model of intra-abdominal sepsis.

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Main Results:

  • The CPC comprises two distinct polysaccharides, PS A and PS B, with charged repeating units.
  • Oppositely charged groups (amino, carboxyl, phosphate) are crucial for both abscess promotion and protection.
  • T cell-dependent immune responses mediate these effects.

Conclusions:

  • The distinct biologic properties of B. fragilis CPC are structurally determined by its charged polysaccharides.
  • These findings provide a structural rationale for CPC's role in host response during sepsis.
  • A specific immune mechanism involving T cells is delineated.