A simple method to make better probes from short DNA fragments
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T Kapros1, A J Robertson, J H Waterborg
1Division of Cell Biology and Biophysics, University of Missouri, Kansas City 64110.
Molecular Biotechnology
|August 1, 1994
Abstract:
A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments.