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Updated: Aug 1, 2026
13:14
Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
Published on: August 10, 2009
Analysis of gene expression by northern blot
1National Institute for Medical Research, Mill Hill, London.
Molecular Biotechnology
|December 1, 1994
Summary
Northern blotting is a key method for analyzing gene expression by measuring RNA levels. This technique involves RNA isolation, electrophoresis, membrane transfer, and hybridization for accurate gene activity monitoring.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Steady-state RNA transcript levels are crucial for monitoring gene activity in various biological systems.
- Several methods exist for RNA quantification, including S1 hybridization, RNase protection, and Northern blotting.
- The choice of RNA analysis method depends on required sensitivity, information type, and experimental system limitations.
Purpose of the Study:
- To detail the methodology of Northern blotting for RNA analysis.
- To provide a comprehensive guide for researchers utilizing Northern blotting techniques.
- To highlight Northern blotting as a reliable method for assessing gene expression.
Main Methods:
- RNA isolation from cell lines or tissues.
- Size fractionation of RNA via gel electrophoresis.
- Transfer of fractionated RNA to a solid membrane support.
- Detection of specific RNA transcripts using nucleic acid hybridization.
- Autoradiography for visualizing hybridization signals.
Main Results:
- Northern blotting enables the detection and quantification of specific RNA transcripts.
- The method allows for the analysis of RNA size and abundance.
- Successful application of Northern blotting provides insights into gene expression patterns.
Conclusions:
- Northern blotting is a versatile and established technique for analyzing RNA steady-state levels.
- The detailed protocol facilitates accurate gene expression monitoring in molecular biology research.
- This method remains valuable for understanding gene activity in both endogenous and introduced gene systems.
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