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Related Experiment Videos

Human gliomas with wild-type p53 express bcl-2

L M Alderson1, R L Castleberg, G R Harsh

  • 1Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Charlestown 02129.

Cancer Research
|March 1, 1995
PubMed
Summary

Human gliomas often overexpress wild-type p53, suggesting a mechanism to evade apoptosis. This study found bcl-2 expression is linked to wild-type p53 in gliomas, indicating a potential role in tumor survival.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Human astrocytomas frequently overexpress wild-type p53, indicating a potential evasion of p53-mediated apoptosis in gliomas.
  • Bcl-2 protein is known to inhibit apoptosis and is expressed in various human cancers.

Purpose of the Study:

  • To investigate the expression of bcl-2 in human gliomas.
  • To determine if bcl-2 expression is associated with wild-type p53 in these tumors.

Main Methods:

  • Immunohistochemical staining for bcl-2 and p53 on 28 paraffin-embedded human gliomas (grades II, III, and IV).
  • Analysis of p53 mutations using single-strand conformation polymorphism and DNA sequencing.

Main Results:

  • Bcl-2 was expressed in 16 out of 28 (57%) gliomas.

Related Experiment Videos

  • Bcl-2 expression showed a significant association with wild-type p53 (P < 0.01).
  • Among gliomas with p53 overexpression, bcl-2 was positive in all wild-type p53 tumors (7/7) but only one mutant p53 tumor (1/7).

    • Conclusions:

    • Bcl-2 is frequently expressed in human gliomas.
    • Bcl-2 expression is more common in gliomas harboring wild-type p53.
    • These findings suggest a role for bcl-2 in the pathogenesis of gliomas with wild-type p53.