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Free radical related myocardial mitochondrial damage following limb ischaemia-reperfusion
E Bastounis1, L Hadjinikolaou, M Pikoulis
1Athens Medical School, First Surgical Department of Laikon Hospital, Greece.
Objective:
The aim was to define the following: (1) if reperfusion of ischaemic limbs could cause myocardial damage; (2) if reactive oxygen metabolites are involved in such possible damage.
Methods:
Ten rats underwent ischaemia-reperfusion of the lower limbs (group A) and 10 underwent the same procedure following treatment with ascorbic acid (group B). Ten rats were used as a control group (group C).
Results:
The incidence of severe myocardial mitochondrial damage and serum malondialdehyde concentrations 30 min after reperfusion were both higher in group A than in groups B and C [8/10, 2/10, and 0/10, p < 0.05 and 7.25 (SEM 0.33), 5.30(0.26), and 4.89(0.23) mumol.litre-1, p < 0.05, respectively].
Conclusions:
Ischaemia-reperfusion of the lower limbs may cause mitochondrial damage in the myocardium and reactive oxygen metabolites could mediate this damage.