[Cytological study on the effect of fibronectin in promoting periodontal regeneration]

Cell cultures and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay were used to observe the different biological effects of fibronectin (FN) on periodontal ligament (PDL) cells and gingival fibroblasts (GF). The results showed that FN has the capacity to accelerate PDL cell multiplication at dose 0.044 mumol/L, and accelerate GF multiplication at does from 0.089 mumol/L to 0.178 mumol/L. It suggests that PDL cells are more susceptible to FN than GF. So, the right amount of FN might promote the PDL cells to occupy root surface firstly and multiply, differentiate, and then to form a new connective tissue attachment.