Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrogenic Na+ transport by Enterococcus hirae Na(+)-ATPase

Y Kakinuma1, K Igarashi

  • 1Faculty of Pharmaceutical Sciences, Chiba University, Japan.

FEBS Letters
|February 13, 1995
PubMed
Summary

Enterococcus hirae

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excretion and uptake of putrescine by the PotE protein in Escherichia coli.

The Journal of biological chemistry·1997
Same author

Molecular mechanism of polyamine stimulation of the synthesis of oligopeptide-binding protein.

The Journal of biological chemistry·1997
Same author

Effects of bisphosphonates on alkaline phosphatase activity, mineralization, and prostaglandin E2 synthesis in the clonal osteoblast-like cell line MC3T3-E1.

Prostaglandins, leukotrienes, and essential fatty acids·1997
Same author

[PotD protein: three-dimensional structure and function].

Seikagaku. The Journal of Japanese Biochemical Society·1997
Same author

Inhibition of a medium chain acyl-CoA synthetase involved in glycine conjugation by carboxylic acids.

Biochemical pharmacology·1996
Same author

A second gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae.

Biochemical and biophysical research communications·1996

Area of Science:

  • Microbiology
  • Biochemistry
  • Cellular Physiology

Background:

  • The F0F1, H(+)-ATPase is crucial for energy metabolism in bacteria.
  • Understanding alternative ion transport mechanisms is vital for bacterial energetics.

Purpose of the Study:

  • To investigate the role of Na(+)-ATPase in membrane potential generation in Enterococcus hirae.
  • To elucidate the conditions and requirements for electrogenic sodium transport.

Main Methods:

  • Utilizing a F0F1, H(+)-ATPase-defective mutant of Enterococcus hirae.
  • Measuring membrane potential (delta psi) under varying pH and ion concentrations (Na+, K+).
  • Comparing wild-type and Na(+)-ATPase mutant strains.

Main Results:

  • A membrane potential (delta psi) of -45 mV was generated in the H(+)-ATPase mutant.
  • This delta psi generation occurred at high pH and required intracellular Na+, but not extracellular K+.
  • Activity was induced by high Na+ culture conditions and absent in Na(+)-ATPase mutants.

Conclusions:

  • The Na(+)-ATPase in Enterococcus hirae functions as an electrogenic sodium pump.
  • This pump contributes to maintaining membrane potential independently of the H(+)-ATPase.

Related Experiment Videos