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Related Experiment Videos

A cDNA from Schizosaccharomyces pombe encoding a putative enolase

J C Jackson1, J M Lopes

  • 1Department of Molecular and Cellular Biochemistry, Loyola University of Chicago, Maywood, IL 60153.

Gene
|February 27, 1995
PubMed
Summary

Researchers isolated an enolase (Eno) gene from the fission yeast Schizosaccharomyces pombe. This gene

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Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Enzymology

Background:

  • Enolase is a crucial enzyme in glycolysis.
  • Understanding enolase function across different species is important.

Purpose of the Study:

  • To isolate and characterize the enolase-encoding gene from Schizosaccharomyces pombe.
  • To investigate the evolutionary conservation and expression patterns of this enolase.

Main Methods:

  • Isolation of a cDNA clone encoding enolase.
  • Deduction of amino acid sequence.
  • Sequence similarity comparisons.
  • Northern blot analysis for gene expression.

Main Results:

  • A 1.4-kb cDNA clone encoding enolase was isolated from Schizosaccharomyces pombe.
  • The deduced amino acid sequence showed significant similarity to enolases from other organisms, particularly Saccharomyces cerevisiae and Candida albicans.
  • Northern blot revealed a single 1.4 kb transcript, most abundant during growth on glucose.

Conclusions:

  • The enolase gene from Schizosaccharomyces pombe is evolutionarily conserved.
  • Gene expression is regulated by carbon source, suggesting a role in glucose metabolism.

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