Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Soluble MHC class I molecules in human body fluids

N Zavazava1, B Wobst, R Ferstl

  • 1Institute of Immunology, Medical School, University of Kiel, Germany.

Journal of Clinical Laboratory Analysis
|January 1, 1994
PubMed
Summary

Soluble human leukocyte antigens (sHLA) are present in various body fluids, with liver as a primary source. Their concentration in serum may increase due to hormonal changes during the menstrual cycle.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exopolysaccharide from Bifidobacterium longum subsp. longum 35624™ modulates murine allergic airway responses.

Beneficial microbes·2018
Same author

Histamine receptor 2 modifies iNKT cell activity within the inflamed lung.

Allergy·2017
Same author

Altered fatty acid metabolism and reduced stearoyl-coenzyme a desaturase activity in asthma.

Allergy·2017
Same author

Ferret lung transplant: an orthotopic model of obliterative bronchiolitis.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012
Same author

Embryonic stem cell-derived T cells induce lethal graft-versus-host disease and reject allogenic skin grafts upon thymic selection.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2011
Same author

Reverse chimerism: stem cells going the other way.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2011

Area of Science:

  • Immunology
  • Biochemistry
  • Human Physiology

Background:

  • Major histocompatibility antigens (MHC) are known for peptide antigen presentation.
  • MHC molecules serve as unique markers for individual body odor in animal models.

Purpose of the Study:

  • To investigate the nature and expression of soluble human MHC class I molecules (sHLA) in human body fluids.
  • To identify the source of low molecular weight sHLA found in serum.

Main Methods:

  • Affinity purification and biochemical analysis of serum class I molecules.
  • SDS-Western blotting of HLA derived from hepatocytes and spleen cells.
  • Detection and quantification of sHLA in serum, plasma, urine, cerebrospinal fluid, and sweat.

Main Results:

Related Experiment Videos

  • Serum class I molecules exhibited a molecular weight range of 45 to 21 kD.
  • The liver was identified as a significant source of low molecular weight sHLA (< 45 kD) in serum.
  • sHLA were detected in serum, plasma, urine, and sweat, but not in cerebrospinal fluid.
  • Serum sHLA levels increased during the first half of the menstrual cycle, particularly in HLA-A24 positive women, suggesting a role for sex hormones.

Conclusions:

  • Soluble human MHC class I molecules are present in various human body fluids, with the liver being a major contributor.
  • Serum sHLA levels are influenced by hormonal fluctuations, specifically during the menstrual cycle.
  • Further research into sHLA may reveal novel biomarkers for physiological states and individual identification.