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A simple method for continuous measurement of cell height during a volume change in a single A6 cell
K Kawahara1, M Onodera, Y Fukuda
1Department of Physiology, Chiba University School of Medicine, Japan.
The Japanese Journal of Physiology
|January 1, 1994
Abstract:
A simple electrophysiological method for continuous monitoring of the height of a single renal cell in culture is described. The procedure consists of placing a patch-electrode on the apical cell surface and monitoring the current deflections during osmotic challenge. The mean increase in cell height by 78% hypotonic solution was 2.0 +/- 0.2 microns.