[Effects of experimental factors on the tinctorial properties of structural elements of the mammalian cerebral cortex (intravital microscopic study)]

Effects of a vital dye--methylene blue--on brain neuronal structures were investigated in the alive preparations of mammal brains by means of contact optical microscope in falling polarization light. Tinctorial properties of neuronal cortical structures were studied under the influence of a number of experimental factors (illumination conditions, dye concentration, temperature regime and oxygen supply to the preparation). A certain dependence in the character of dyeing and bleaching neuronal cells on the factors mentioned above was stated. Conditions for appearance of the alternating photodynamical effect and measures to prevent it were clarified. Practical recommendations were given for application methylene blue in prevital morphological investigations of mammal central nervous system.