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Subzero thermal analysis of human stratum corneum
H Tanojo1, J A Bouwstra, H E Junginger
1Division of Pharmaceutical Technology, Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands.
Pharmaceutical Research
|November 1, 1994
Summary
This study reveals a new thermal transition in human stratum corneum at -9°C, distinct from water. This subzero transition is attributed to the skin
Area of Science:
- Biophysics
- Dermatology
- Materials Science
Background:
- The stratum corneum (SC) is the outermost layer of the skin, crucial for barrier function.
- Understanding SC thermal behavior is vital for drug delivery and cosmetic formulation.
- Previous studies identified thermal transitions related to SC structure and hydration.
Purpose of the Study:
- To investigate the thermal behavior of human stratum corneum using differential thermal analysis (DTA).
- To identify and characterize previously unreported thermal transitions.
- To elucidate the molecular origin of a novel subzero thermal transition.
Main Methods:
- Differential thermal analysis (DTA) of human stratum corneum.
- Analysis conducted across a temperature range of -130°C to 120°C.
- Stratum corneum samples were analyzed after lipid extraction and treatment with propylene glycol and oleic acid/propylene glycol solutions.
Main Results:
- Identified known thermal transitions at 40°C, 70°C, 85°C, and 100°C.
- Discovered a novel subzero transition at approximately -9°C (264 K) in both hydrated and dehydrated SC.
- This subzero transition was differentiated from the water peak observed in hydrated samples.
Conclusions:
- The subzero transition at -9°C is a distinct thermal event in the human stratum corneum.
- This transition is linked to the presence of low-melting lipid components within the stratum corneum.
- These findings enhance our understanding of SC lipid behavior and thermal properties.