Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A program for analyzing enzyme rate data obtained from a microplate reader

S P Brooks1

  • 1Nutrition Research Division, Health Canada 3W Banting Research Centre, Ottawa, Ontario.

Biotechniques
|December 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complex cognitive and motivational deficits precede motor dysfunction in the zQ175 (190 CAG repeat) Huntington's disease model.

Experimental neurology·2025
Same author

Five choice serial reaction time performance in the HdhQ92 mouse model of Huntington's disease.

Brain research bulletin·2011
Same author

Gene expression and behaviour in mouse models of HD.

Brain research bulletin·2011
Same author

Early onset deficits on the delayed alternation task in the Hdh(Q92) knock-in mouse model of Huntington's disease.

Brain research bulletin·2011
Same author

Operant-based instrumental learning for analysis of genetically modified models of Huntington's disease.

Brain research bulletin·2011
Same author

Rule learning, visuospatial function and motor performance in the Hdh(Q92) knock-in mouse model of Huntington's disease.

Behavioural brain research·2009
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

The microplate analysis (MPA) program offers enhanced control for analyzing kinetic data from microplate readers. This software facilitates precise enzyme velocity calculations and data visualization for detailed reaction analysis.

Area of Science:

  • Biochemistry
  • Computational Biology
  • Enzymology

Background:

  • Microplate readers generate extensive kinetic data.
  • Analyzing this data for enzyme kinetics can be complex.
  • Accurate enzyme velocity determination is crucial in biochemical assays.

Purpose of the Study:

  • To introduce a novel computer program, the microplate analysis (MPA) program.
  • To enhance the manipulation and analysis of kinetic data from microplate readers.
  • To provide precise calculation of enzyme velocities and reaction rates.

Main Methods:

  • The MPA program processes kinetic data from microplate readers.
  • Data can be visualized as an 8 x 12 grid or individual enzyme progress curves.
  • Enzyme velocities are calculated using linear or robust regression analysis.

Related Experiment Videos

Main Results:

  • The MPA program allows detailed data manipulation in single curve mode.
  • Users can control data point ranges for regression analysis per well.
  • The program enables precise determination of maximum, minimum, or initial reaction rates.

Conclusions:

  • The MPA program provides significant user control over kinetic data presentation and calculation.
  • Its well-specific range settings offer advanced analytical flexibility.
  • This tool is valuable for researchers working with microplate-based kinetic assays.