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Synthesis and primer properties of oligonucleotides containing 3'-deoxypsicothymidine units, labeled with fluorescein
A Guzaev1, E Azhayeva, J Hovinen
1Department of Chemistry, University of Turku, Finland.
Bioconjugate Chemistry
|November 1, 1994
Abstract:
Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.