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Related Experiment Videos

Proton ATPases and urinary acidification

J G Kleinman1

  • 1Medical College of Wisconsin, Section of Nephrology, Milwaukee.

Journal of the American Society of Nephrology : JASN
|November 1, 1994
PubMed
Summary
This summary is machine-generated.

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Kidney acidification involves proton pumps, but the vacuolar H+ ATPase may not be solely responsible for all urinary acidification. A gastric-type H(+)-K+ ATPase also plays a role.

Area of Science:

  • Nephrology
  • Cell Biology
  • Physiology

Background:

  • Urinary acidification is crucial for kidney function and is mediated by proton (H+) transport.
  • A proton ATPase has been identified as a key transporter in this process, particularly in the proximal tubule and collecting duct.

Purpose of the Study:

  • To review unresolved questions regarding the distribution, regulation, and pathophysiologic role of the proton transporter in urinary acidification.
  • To evaluate the extent to which the vacuolar H+ ATPase contributes to urinary acidification.
  • To examine the evidence for a gastric-type H(+)-K+ ATPase in the kidney.

Main Methods:

  • Literature review of studies on proton transport in the kidney.
  • Analysis of evidence for vacuolar H+ ATPase presence and function.

Related Experiment Videos

  • Review of studies investigating H(+)-K+ ATPase activity and inhibition.

    • Main Results:

    • The vacuolar H+ ATPase likely does not account for all urinary acidification, as evidence suggests acidification occurs at sites lacking this transporter.
    • Compelling evidence supports the presence of a gastric-type H(+)-K+ ATPase in the kidney, sensitive to specific inhibitors.
    • Uncertainties persist regarding transporter specificity and conflicting molecular biology data.

    Conclusions:

    • The vacuolar H+ ATPase is a significant mediator of urinary acidification but probably not the sole contributor.
    • A gastric-type H(+)-K+ ATPase is likely involved in kidney acidification, though further research is needed to clarify its precise role and overcome current limitations.